Product Info Summary
SKU: | A00085-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PCSK9 Antibody Picoband™
View all Proprotein Convertase 9/PCSK9 Antibodies
SKU/Catalog Number
A00085-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCSK9 Antibody Picoband™ catalog # A00085-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCSK9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00085-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse PCSK9 recombinant protein (Position: Q182-H560).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00085-2 is reactive to Pcsk9 in Mouse, Rat
Applications
A00085-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
74 kDa
Calculated molecular weight
74.823kDa
Background of Proprotein Convertase 9/PCSK9
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. PCSK9 can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PCSK9/Pcsk9 using anti-PCSK9/Pcsk9 antibody (A00085-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9/Pcsk9 antigen affinity purified polyclonal antibody (Catalog # A00085-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9/Pcsk9 at approximately 74KD. The expected band size for PCSK9/Pcsk9 is at 74KD.
Protein Target Info & Infographic
Gene/Protein Information For Pcsk9 (Source: Uniprot.org, NCBI)
Gene Name
Pcsk9
Full Name
Proprotein convertase subtilisin/kexin type 9
Weight
74.823kDa
Superfamily
peptidase S8 family
Alternative Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pcsk9, check out the Pcsk9 Infographic
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In this infographic, you will see the following information for Pcsk9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCSK9 Antibody Picoband™ (A00085-2)
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No publications found for A00085-2
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